Journal: Signal Transduction and Targeted Therapy

Article Title: Targeting coronaviral inflammation: aptamer-based strategies for emerging threats

doi: 10.1038/s41392-025-02570-8

Figure Lengend Snippet: Design and performance of a circular trivalent chimera for SARS-CoV-2 via NApt8-3. a Schematic illustration of the principle underlying efficient targeted delivery of circSASON to suppress SARS-CoV-2 (WT) replication and inflammation. CorelDraw was used to generate these images. b Flow cytometry analysis of SApt-dependent and SARS-CoV-2 infection-mediated delivery of circSASON into hACE2-HEK293T cells. Blank (without any treatment) and RS were used as negative controls. SApt as a positive control. The upper panel shows the GFP signal from the SARS-CoV-2 Spike (WT) Fluc-GFP pseudovirus. The lower panel displays the Cy3 signal from the Cy3-labeled oligonucleotides (RS, SApt or cirSASON). c Representative fluorescence confocal images of hACE2-HEK293T cells infected with SARS-CoV-2 pseudovirus (5 × 10 4 TCID50 mL −1 ) preincubated with the indicated 200 nM Cy3-labeled oligonucleotides ( n = 3). S-pseudovirus spike-GFP: SARS-CoV-2 spike (WT) Fluc-GFP pseudovirus. Scale bars represent 10 µm. d Representative fluorescence confocal images of HEK293T and hACE2-HEK293T cells incubated with SARS-CoV-2 pseudovirus (5 × 10 4 TCID50 mL −1 ) and Cy3-labeled circSASON (200 nM). Scale bars represent 10 µm

Article Snippet: Calu-3 cells (CL-0054) were obtained from Procell Life Science& Technology Co., Ltd (ATCC, HTB-55 BCRJ, 0264).hACE2-HEK293T cells were cultured in DMEM medium (Gibco) supplemented with 100 U/mL Penicillin-Streptomycin, 2 μg/mL Puromycin and 10% (v/v) fetal bovine serum (ExCell Bio), in a humidified atmosphere containing 5% CO 2 incubator at 37 °C.

Techniques: Flow Cytometry, Infection, Positive Control, Labeling, Fluorescence, Incubation